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主营:细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂
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MD Biosciences成立于1991年,总部位于瑞士,在美国、加拿大和英国设有办事处。旗下MD Bioproducts部门主要产品涉及肿瘤、心血管、细胞培养、内分泌、炎症、代谢疾病、神经科学、疼痛、肾脏/肝脏功能等领域。产品包括抗体(软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂(胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂(关节炎诱导抗体混合、弗氏佐剂、胶原蛋白II等)、ELISA试剂盒、同型对照、天然蛋白以及重组蛋白。
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MD Bioproducts/Collagen Type I and III, canine/8052008/10 mg

  • 产品编号: 8052008
  • 美  元  价: $0.00
  • 会  员  价: 待定
  • 品       牌: MD Bioproducts
  • 产       地: 美国
  • 公       司: MD Bioproducts
  • 产品分类: ref="." class="index">首页 > MD Bioproducts/Collagen Type I and III, canine/8052008/10 mg
  • 公司分类: natural and recombinant proteins
:10 mg
MD Bioproducts/Collagen Type I and III, canine/8052008/10 mg
  • MD Bioproducts/Collagen Type I and III, canine/8052008/10 mg
商品介绍
Overview: 

Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal BIOLOGical scaffold to promote more in vivo-like cellular morphology and function.

 

Type III collagen is the second most abundant collagen in tissues and is found most commonly in tissues exhibiting elastic properties such as skin, lungs, intestinal walls and walls of blood vessels. It is a homotrimer comprised of three alpha-1 chains and resembles other fibrillar collagens in structure and function. It is synthesized as procollagen, similary to collagen I, but the N-terminal propeptide remains attached in the mature fibrillar type III form.

 

Symbols/Related Terms:

 

  • COL1A1
  • COL1A2
  • COL3A1
  • Collagen type III, alpha 1
  • osteogenesis imperfecta

 

 

Data/Specifications: 

Purity: 

Canine collagen type I and III - 98%

Canine Collagen (other types) - <1%

Non-collagen proteins - below 0.5%

 

Form: 10 mg lyophilized, salt-free

 

Purification: Partial pepsin digestion in acidic conditions and differential salt precipitation.

 

Source: Canine tissue. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I and III were purified by using differential salt precipitation.

 

Reconstitution: Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ABIlity to form microfibrils.

 

Storage: Collagen dissolved in acetic acid is stable at 4 °C for 1 month. Lyophilized collagen long term storage (2 years) at -20°C or lower.

How_To_Use:

Key Applicatons:

  • Coating material for cell culture studies. 

Note: May not be suitable for 3-D gel formation.

品牌介绍

MD Biosciences成立于1991年,总部位于瑞士,在美国、加拿大和英国设有办事处。旗下MD Bioproducts部门主要产品涉及肿瘤、心血管、细胞培养、内分泌、炎症、代谢疾病、神经科学、疼痛、肾脏/肝脏功能等领域。产品包括抗体 (软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂 (关节炎诱导抗体混合、弗氏佐剂、胶原蛋白II等)、ELISA试剂盒、同型对照、天然蛋白以及重组蛋白。

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