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MD Biosciences成立于1991年,总部位于瑞士,在美国、加拿大和英国设有办事处。旗下MD Bioproducts部门主要产品涉及肿瘤、心血管、细胞培养、内分泌、炎症、代谢疾病、神经科学、疼痛、肾脏/肝脏功能等领域。产品包括抗体 (软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂 (关节炎诱导抗体混合、弗氏佐剂
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MD Bioproducts/ADAMTS5 Human Recombinant Protein, truncated, His-tagged/5044014/5 µg

MD Bioproducts/ADAMTS5 Human Recombinant Protein, truncated, His-tagged/5044014/5 µg
  • MD Bioproducts/ADAMTS5 Human Recombinant Protein, truncated, His-tagged/5044014/5 µg
商品介绍
Overview: 

ADAMTS(adisintegrinandmetalloproteinasewiththrombospondinmotif)aremultidomainextracellularproteinases,containingatleastonethrombospondintype-1repeat[1].Theenzymesareinvolvedinprocessingofextracellularmatrixproteinsandproteinsinthecirculation.ADAMTS5wasfirstpurifiedfrombovinenasalcartilageconditionedmediaandhumanADAMTS5CDNAwasclonedfromahumanlivercDNAlibrary[2].ADAMTS5consistsofaprodomain,acatalyticdomain,adisintegrindomain,athrombospondintype-1motif,aCys-richregionfollowedbyaspacersequenceandthrombospondinsubmotifs.Theprodomainismostlikelycleaved-offbyafurin-typeenzymebeforeADAMTS5isreleasedfromcells.



ADAMTS5isexpressedconstitutivelyinsynovium[3]anditdegradesaggrecan,themajorproteoglycanofarticularcartilage[4].LikeADAMTS4,anothermemberoftheADAMTS-family,ADAMTS5cleavesaggrecanat5sites[4].Fourcleavagesitesarelocatedinthechondroitinsulfate-richregionbetweenaggrecanglobulardomainsG2andG3,whileonesiteiscontainedintherod-shapedpolypeptidebetweenglobulardomainsG1andG2.Inaddition,ADAMTS5cleavesonemoresiteinthechondroitinsulfate-richregionthatisnotcleavedbyADAMTS4[4].ADAMTS5isinhibitedbyTIMP3[5,6]and2-macroglobulin[7].

Data/Specifications: 

Purity:AsjudgedfromSDS-PAGEtruncatedADAMTS5represents50%oftotalproteinofthepreparation.Theproteinconcentrationis0.1mg/ml.

 

SpecificActivity:AggrecanaseactivityoftheADAMTS5preparationisdeterminedwithrecombinantaggrecaninterglobulardomain(Aggrecan-IGDfromMDBiosciences).ADAMTS4hydrolyzesthe“aggrecanase”sitewithinthisdomain(peptidebondE373-A374inhumanaggrecan). Therecombinantsubstrateisincubatedataconcentrationof0.1μMwithADAMTS5in50mMTris-HCl,pH7.5,150mMNaCl,5mMCaCl2,1μMleupeptin,1μMpepstatin,1mMPefabloc,0.05%Brij35for15minat37°C.AseriesofADAMTS5dilutionsrangingfrom103-to105-foldistested.Substratecleavageatthe“aggrecanase”-siteisestimatedfromtheappearanceofthehydrolysisfragmentwiththenovelN-terminusARGSVIL.Thefragmentisquantifiedwithtwomonoclonalantibodies,onedirectedagainsttheneoepitopeARGSVIL,theotheragainstthesequenceC-terminalfromtheneoepitope(InviLISAAggrecanaseActivityAssay).Underthespecifiedconditionsthehydrolysisrateis>0.5nmoleshydrolyzedsubstrate/minxmlADAMTS5preparationor>5nmoleshydrolyzedsubstrate/minxmg.

 

Inhibitors:ADAMTS5isinhibitedbytissueinhibitorofmatrixmetalloproteinases3(TIMP3)andby2-macroglobulin(seesection3).EnzymeactivityisalsosuppressedbychelatorsofdivalentcationsasEDTAandbysyntheticmetalloproteinaseinhibitors.

 

StABIlity&Storage:RecombinantADAMTS5isstableuntiltheexpirydategivenonthelabelwhenstoredat-70°C.Theenzymecanbekeptat-20°Cforseveralweeks.Repeatedfreezingandthawingshouldbeavoided.

 

Applications:RecombinantADAMTS5isusedtostudythedegradationofextracellularmatrixproteoglycans,toscreenforinhibitorsofADAMTS5andtocharacteriseinhibitoractions.Theenzymepreparationcanalsobeutilizedinenzymaticandimmunochemicalassays.

品牌介绍

MD Biosciences成立于1991年,总部位于瑞士,在美国、加拿大和英国设有办事处。旗下MD Bioproducts部门主要产品涉及肿瘤、心血管、细胞培养、内分泌、炎症、代谢疾病、神经科学、疼痛、肾脏/肝脏功能等领域。产品包括抗体 (软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂 (关节炎诱导抗体混合、弗氏佐剂、胶原蛋白II等)、ELISA试剂盒、同型对照、天然蛋白以及重组蛋白。

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