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MD Biosciences成立于1991年,总部位于瑞士,在美国、加拿大和英国设有办事处。旗下MD Bioproducts部门主要产品涉及肿瘤、心血管、细胞培养、内分泌、炎症、代谢疾病、神经科学、疼痛、肾脏/肝脏功能等领域。产品包括抗体 (软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂 (关节炎诱导抗体混合、弗氏佐剂
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MD Bioproducts/Matrix Metalloproteinase 13 (MMP-13), His-tagged/5044010/10 µg

MD Bioproducts/Matrix Metalloproteinase 13 (MMP-13), His-tagged/5044010/10 µg
  • MD Bioproducts/Matrix Metalloproteinase 13 (MMP-13), His-tagged/5044010/10 µg
商品介绍
Overview: 

Matrixmetalloproteinases(MMP)areZn2+-andCa2+-dependentendopeptidases[1].MainsubfamiliesofMMPareCollagenases,gelatinases,stromelysinsandmembrane-typematrixmetalloproteinases[2].Threehomologouscollagenaseshavebeenidentifiedinhumantissues:interstitialcollagenase,neutrophilcollagenaseandcollagenase-3.Thethreeenzymescleavefibrillarcollagensatasinglesite,generatingfragmentsofapproximately¾and¼thesizeoftheoriginalmolecules.


Procollagenase-3consistsof452aminoacidswithacalculatedMrof51.680Da[3].DuetoN-linkedglycosylationtheapparentMrisabout60.000Da[4].Withintheproteinthefollowingdomainsandsequenceregionscanbedistinguished[3,4]:anN-terminalpropeptide,whichconferslatencytotheproenzyme,aCa2+-andZn2+-ionsbindingcatalyticdomain,ahingeregion,andaC-terminalhemopexin-likedomain.

Procollagenase-3canbeactivatedbyproteasesasstromelysin[4],gelatinaseA,membranetype1matrixmetalloproteinaseandplasmin[5]orbyincubationwithorganomercurials(e.g.APMA)[4].

Activecollagenase-3beginswiththeN-terminalsequenceYNVFPRTL[4].Collagenase-3hydrolyzestypeIIcollagen5-to6-timesfasterthantypeIandtypeIIIcollagens.TheenzymeexhibitsalsohighactivitytowardsgelatinanditdegradesSERPINSas1-antichymotrypsinandplaminogenactivatorinhibitor-2[4].Collagenase-3isinhibitedina1:1stoichiometricfashionbyTIMP-1,TIMP-2andTIMP-3.Collagenase-3isexpressedduringfetalbonedevelopment[6]. Inadulthumantissuescollagenase-3hasbeendetectedonlyinpathologicalconditions:inmalignanttumors[3],inchroniculcers[7],inarthriticcartilage[8]andsynovium[9].

Data/Specifications: 

Purity:Recombinantprocollagenase-3appearsasasingleproteinbandofabout60.000DainSDS-PAGE(>95%oftotalprotein).Duetoautoproteolyticactivityminorbandsofactivatedcollagenase-3maybevisIBLeintheenzymepreparation.

 

SpecificActivity:Thespecificactivityofcollagenase-3is≥200mU/mg.1Uistheactivitythathydrolyzes1molpeptide(7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L-2,3-diaminopropionyl)-Ala-Arg-NH2(Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg)within1minundertheassayconditionsdescribedbelow.

 

Inhibitors:Collagenase-3isinhibitedbytissueinhibitorsofmatrixmetalloproteinases(TIMP)andbychelatorsofdivalentcationsasEDTAoro-phenanthroline.

 

StABIlity&Storage:Recombinantprocollagenase-3tableuntiltheexpirydategivenonthelabelifstoredat-70°C.Theproenzymecanbekeptat-20°Cforseveralweeks.Repeatedfreezingandthawingshouldbeavoided.

 

Applications:Recombinantprocollagenase-3canserveasantigenstandardinimmunochemicalanalyses. Theactiveenzymemaybeusedtostudythedegradationofextracellularmatrixproteins,toscreeninhibitorsofmatrixmetalloproteinasesandtocharacterizeinhibitoractions.

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MD Biosciences成立于1991年,总部位于瑞士,在美国、加拿大和英国设有办事处。旗下MD Bioproducts部门主要产品涉及肿瘤、心血管、细胞培养、内分泌、炎症、代谢疾病、神经科学、疼痛、肾脏/肝脏功能等领域。产品包括抗体 (软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂 (关节炎诱导抗体混合、弗氏佐剂、胶原蛋白II等)、ELISA试剂盒、同型对照、天然蛋白以及重组蛋白。

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