请使用支持JavaScript的浏览器! mdbioproducts-natural and recombinant proteins-MD Bioproducts/Matrix Metalloproteinase 13 (MMP-13), His-tagged/5044010/10 µg
主营:细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂
banner
当前位置: 首页 > 产品中心 > natural and recombinant proteins > MD Bioproducts/Matrix Metalloproteinase 13 (MMP-13), His-tagged/5044010/10 µg
公司介绍
MD Biosciences成立于1991年,总部位于瑞士,在美国、加拿大和英国设有办事处。旗下MD Bioproducts部门主要产品涉及肿瘤、心血管、细胞培养、内分泌、炎症、代谢疾病、神经科学、疼痛、肾脏/肝脏功能等领域。产品包括抗体(软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂(胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂(关节炎诱导抗体混合、弗氏佐剂、胶原蛋白II等)、ELISA试剂盒、同型对照、天然蛋白以及重组蛋白。
问答列表

    暂无问答

MD Bioproducts/Matrix Metalloproteinase 13 (MMP-13), His-tagged/5044010/10 µg

  • 产品编号: 5044010
  • 美  元  价: $0.00
  • 会  员  价: 待定
  • 品       牌: MD Bioproducts
  • 产       地: 美国
  • 公       司: MD Bioproducts
  • 产品分类: ref="." class="index">首页 > MD Bioproducts/Matrix Metalloproteinase 13 (MMP-13), His-tagged/5044010/10 µg
  • 公司分类: natural and recombinant proteins
:10 µg
MD Bioproducts/Matrix Metalloproteinase 13 (MMP-13), His-tagged/5044010/10 µg
  • MD Bioproducts/Matrix Metalloproteinase 13 (MMP-13), His-tagged/5044010/10 µg
商品介绍
Overview: 

Matrix metalloproteinases (MMP) are Zn2+- and Ca2+- dependent endopeptidases [1]. Main subfamilies of MMP are Collagenases, gelatinases, stromelysins and membrane-type matrix metalloproteinases [2]. Three homologous Collagenases have been identified in human tissues: interstitial Collagenase, neutrophil Collagenase and Collagenase-3. The three enzymes cleave fibrillar collagens at a single site, generating fragments of approximately ¾ and ¼ the size of the original molecules.


ProCollagenase-3 consists of 452 amino acids with a calculated Mr of 51.680 Da [3]. Due to N-linked glycosylation the apparent Mr is about 60.000 Da [4]. Within the protein the following domains and sequence regions can be distinguished [3, 4]: an N-terminal propeptide, which confers latency to the proenzyme, a Ca2+- and Zn2+- ions binding catalytic domain, a hinge region, and a C-terminal hemopexin-like domain.

ProCollagenase-3 can be activated by proteases as stromelysin [4], gelatinase A, membranetype 1 matrix metalloproteinase and plasmin [5] or by incubation with organomercurials (e.g. APMA) [4].

Active Collagenase-3 begins with the N-terminal sequence YNVFPRTL [4]. Collagenase-3 hydrolyzes type II collagen 5- to 6-times faster than type I and type III collagens. The enzyme exhibits also high activity towards gelatin and it degrades SERPINS as 1-antichymotrypsin and plaminogen activator inhibitor-2 [4]. Collagenase-3 is inhibited in a 1:1 stoichiometric fashion by TIMP-1, TIMP-2 and TIMP-3. Collagenase-3 is expressed during fetal bone development [6].  In adult human tissues collagenase-3 has been detected only in pathological conditions: in malignant tumors [3], in chronic ulcers [7], in arthritic cartilage [8] and synovium [9].

Data/Specifications: 

Purity: Recombinant procollagenase-3 appears as a single protein band of about 60.000 Da in SDS-PAGE (> 95% of total protein). Due to autoproteolytic activity minor bands of activated collagenase-3 may be visIBLe in the enzyme preparation.

 

Specific Activity: The specific activity of collagenase-3 is ≥ 200 mU/mg. 1 U is the activity that hydrolyzes 1 mol peptide (7-methoxycoumarin-4-yl) acetyl-Pro-Leu-Gly-Leu-(3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl)-Ala-Arg-NH2 (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg) within 1 min under the assay conditions described below.

 

Inhibitors: Collagenase-3 is inhibited by tissue inhibitors of matrix metalloproteinases (TIMP) and by chelators of divalent cations as EDTA or o- phenanthroline.

 

StABIlity & Storage: Recombinant procollagenase-3 table until the expiry date given on the label if stored at -70 °C. The proenzyme can be kept at -20 °C for several weeks. Repeated freezing and thawing should be avoided.

 

Applications: Recombinant procollagenase-3 can serve as antigen standard in immunochemical analyses.  The active enzyme may be used to study the degradation of extracellular matrix proteins, to screen inhibitors of matrix metalloproteinases and to characterize inhibitor actions.

品牌介绍

MD Biosciences成立于1991年,总部位于瑞士,在美国、加拿大和英国设有办事处。旗下MD Bioproducts部门主要产品涉及肿瘤、心血管、细胞培养、内分泌、炎症、代谢疾病、神经科学、疼痛、肾脏/肝脏功能等领域。产品包括抗体 (软骨蛋白聚糖、胶原蛋白、激素、神经药物)、细胞培养相关试剂 (胶原蛋白、细胞增殖检测、支原体PCR检测)、疾病诱导试剂 (关节炎诱导抗体混合、弗氏佐剂、胶原蛋白II等)、ELISA试剂盒、同型对照、天然蛋白以及重组蛋白。

自营商城图标
厂家直采
全球直采模式 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔
在线客服
客服电话

4000-520-616

微信交流